PCR-ELISA Protocol
Experiment Tags: PCR Amplification, Probe Hybridization, ELISA Assay
Experiment Summary: This experiment describes the procedure of PCR-ELISA experiment based on immunological method to detect PCR products.
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Protocols
Protocol
Protocol
Converting Adherent Cells to Suspension Cells (Serum Dilution) Protocol
Experiment Tags: Cell, Adherent Cells, Suspension Cells
Experiment Summary: If the cell viability is >85% and the generation time is similar to that observed with the original medium, it suggests that the cells adapt to growth in current condition.
Protocol
Determining Antibiotic Killing Curve for Stable Cell Line Generation Protocol
Experiment Tags: Cell, Cell Line Generation
Experiment Summary: Since mammalian cells sensitivity to antibiotics varies from one cell type to another, it is essential to determine the minimum concentration of antibiotic required to kill non-transfected or non-transduced cells by separate kill curve experiments.
Protocol
Adenovirus Construction by Homologous Recombination in Bacteria Protocol
Experiment Tags: Adenovirus
Experiment Summary: In our protocol, the recombination between the shuttle plasmid and the adenovirus genome contained in the backbone plasmid is performed in the E. coli strain. Positive recombinants are selected by resistance to an antibiotic.
Protocol
Generation of Stable Cell Lines Using Lentivirus Protocol
Experiment Tags: Cell, Cell Line Generation, Lentivirus
Experiment Summary: This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.
Protocol
Generation and Amplification of Recombinant Adenovirus Protocol
Experiment Tags: Adenovirus, Recombinant Adenovirus
Experiment Summary: Conventional approaches to producing small volumes of viral vectors involve culturing cells in stationary and adherent cultures.
Protocol
LPS Extraction Protocol
Experiment Tags: Lipopolysaccharide, LPS Extraction
Experiment Summary: Here, we describe a hot aqueous-phenol method for the isolation and purification of LPS from gram-negative bacterial cells.
Protocol
BCA Library Construction Protocol
Experiment Tags: BCA Library, Library, Library Construction
Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.
Protocol
cDNA Library Construction Protocol
Experiment Tags: cDNA Library, Library, Library Construction
Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).
Protocol
Viral Titering-TCID50 Assay Protocol
Experiment Tags: Viral Titering, TCID50 Assay
Experiment Summary: A TCID50 was found of greatest value when lack of experimental material or costs of analysis precludes extensive replicate titer determinations but good estimates of titers and treatment differences are essential.
For research use only. Not intended for any clinical use.
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