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Experiment Tags: sgRNA, CRISPR
Experiment Summary: Selection of sgRNAs for an experiment needs to balance two aspects: maximizing on-target activity while minimizing off-target activity, which sounds obviously but can often require thorough decisions.
Experiment Tags: Cell, Cell Line
Experiment Summary: The fundamental principle is to add regulatory elements to the upstream of the target gene through artificial construction, so that genes can be transcribed and translated efficiently under controlled conditions.
Experiment Tags: Fosmid Library, Library, Library Construction
Experiment Summary: The fosmid system has been widely used in the construction of large-insert genomic libraries. Fosmid clones are similar in insert size comparing with cosmid clones and smaller than that in BAC and yeast artificial chromosomes (YAC).
Experiment Tags: CRISPR, CRISPR Plasmid
Experiment Summary: The CRISPR/Cas9 system has two critical parts: sgRNA sequence and Cas9 sequence. These two sequences could be cloned into separated plasmids or combined into one plasmid, each format has its own set of advantages and shares a similar delivery procedure.
Experiment Tags: Codon Optimization, Codon
Experiment Summary: Codon optimization is one of the key steps in achieving the high level expression of target gene. There are some key factors for consideration including transcription efficiency, translation efficiency, gene synthesis and protein folding.
Experiment Tags: Baculovirus, Baculovirus expression vector system, BEVS
Experiment Summary: BEVS is recognized as a powerful tool for the expression of the large-scale protein on the strength of convenient process, high expression level and the function of post-translational modifications.
Experiment Tags: ATAC-seq, Robust Tn5 Transposase, Tn5 Transposase
Experiment Summary: Sequence the library and correlate reads with open and closed chromatin. When sequencing ATAC-seq libraries, sequencing primers and reagents must be used.
Experiment Tags: Adenoviral Vectors, Adenovirus
Experiment Summary: The adenoviral vector is part of a bacterial plasmid. In order to release the adenoviral ends, it is necessary to digest with Pac I. The best method is to digest 12 µg of the plasmid in 100 µl total volume for 3 hours.
