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Experiment Tags: Gene Transfer, Central Nervous System (CNS), Adenoviral Vectors
Experiment Summary: This protocol describes a procedure for the direct injection of adenovirus into the CNS using stereotaxic guidance.
Experiment Tags: Cytotrap System, Yeast Two-Hybrid Screen, Protein Interaction
Experiment Summary: This protocol describes the construction of a cDNA library from the model plant Arabidopsis and a procedure for screening interaction proteins of AtSR1/CAMTA3, a Ca2+/CaM-regulated transcription factor from this library.
Experiment Tags: Retroviral Vectors, Purification, Injection.
Experiment Summary: This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.
Experiment Tags: Lentiviral Transduction, Mouse Induced Pluripotent Stem Cells.
Experiment Summary: This protocol describes a method for generating and characterizing iPS cells from mouse embryonic fibroblasts using a lentiviral transduction system.
Experiment Tags: AAV Vectors, Intracranial Stereotactic Injection, AAV Delivery
Experiment Summary: This protocol describes how to perform an aseptic stereotaxic survival surgery to deliver AAV vectors to the brain of a mouse.
Experiment Tags: Flavivirus, Fluorescence, NS2B-NS3 (NS2B-NS3), Live-cell imaging, Reporter cells, Plaque assay, Image analysis
Experiment Summary: This protocol describes methods for generating, selecting and implementing stable BHK-21 cells that express molecular reporters encoding viral genes suitable for monitoring viral infection by live cell imaging.
Experiment Tags: Cell Immortalization, Lentiviral Transduction
Experiment Summary: The protocol introduces the definition of cell immortalization and commonly used viral transfection methods, and describes in detail the steps to generate immortalized cells using prefabricated cell immortalization lentiviral particles.
Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy
Experiment Summary: This protocol describes a new method, the AAV-Cpf1 KIKO system, using a combination of viral and non-viral approaches to generate a stable CAR-T with homology-directed repair (HDR) knock-in and immune checkpoint knockout at high efficiency in one step.
Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline
Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.
Experiment Tags: Lentivirus, Tumor, Lymphoma, Luciferase, GFP, Mouse
Experiment Summary: In this protocol, we describe methods for the production of stable luciferase expressing tumor cell lines by lentiviral transduction.
