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Experiment Tags: Plasmid DNA, Transformation, Drug-Resistant Plasmids
Experiment Summary: This experiment describes the method of transferring plasmid DNA molecules into E. coli and how to screen cell clones with plasmid DNA, i.e. transformants.
Experiment Tags: Genomic, Overexpression, Library
Experiment Summary: This protocol might work for searching complementation of other gene deletion mutants that have a good selective phenotype.
Experiment Tags: Particle bombardment, Maize embryos, Promoter expression, Quantitative analysis
Experiment Summary: This protocol describes the entire process of transient transformation of maize embryos using particle bombardment.
Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic , Transformation, Prostate
Experiment Summary: This protocol describes how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.
Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
Experiment Tags: Circular RNA, Backsplice junction, Divergent primer, RT-PCR (RT-PCR), RNase R (RNase R)
Experiment Summary: This protocol describes the design of divergent primers which face away from each other on the linear RNA, so that they can only amplify the circRNAs, and not the linear RNAs with the same sequence.
Experiment Tags: Neuron-preferential, Lentiviral vectors
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
Experiment Tags: Arabidopsis Thaliana, Transgenic
Experiment Summary: This experiment describes the use of the flower immersion method to transfer target genes into Arabidopsis thaliana using Agrobacterium mediated transfer.
Experiment Tags: Labeling,Microarray Analysis
Experiment Summary: This experiment describes how to perform labeling procedure for microarray analysis.
Experiment Tags: Green Fluorescent Protein, Zebrafish, Transgenic
Experiment Summary: In this experiment, EGFP-containing plasmid was introduced into zebrafish to observe its expression in animals. In zebrafish, green fluorescent protein could be observed under fluorescence microscope from proto-intestinal embryo to emergence stage.
